The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 20, 2002
Filed:
Oct. 31, 2000
Applicant:
Inventors:
Masaru Honjo, Chiba, JP;
Naokazu Naitoh, Chiba, JP;
Hiroshi Uchida, Chiba, JP;
Daisuke Mochizuki, Chiba, JP;
Kazuya Matsumoto, Chiba, JP;
Assignee:
Mitsui Chemicals, Inc., , JP;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 1/509 ; C12P 2/106 ; C07K 5/00 ; C07K 1/400 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12N 1/509 ; C12P 2/106 ; C07K 5/00 ; C07K 1/400 ; C07H 2/104 ;
Abstract
A DNA encoding 20K hGH is connected directly to a gene encoding OppA protein secretion signal, or a modified form thereof, and a DNA encoding signal peptidase 1 to construct a recombinant plasmid, is transformed by the plasmid and cells of the resulting transformant strain are cultured for secretory production of the 20K hGH in the periplasm. This method enables efficient secretory production of 20K hGH and easy isolation and purification of 20K hGH from the periplasm fraction because the level of impure proteins in the periplasm is low.