The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 29, 2000
Filed:
Nov. 13, 1995
Menashi A Cohenford, West Warwick, RI (US);
Prashant S Bhandare, Arlington, MA (US);
Frederick R Cahn, Princeton, NJ (US);
Krishnaswamy Krishnan, Norwell, MA (US);
Basil Rigas, White Plains, NY (US);
Bio-Rad Laboratories, Inc., Hercules, CA (US);
Abstract
This invention discloses a method to identify premalignant and malignant stages of cervical cancer from an infrared (IR) spectrum of exfoliated cervical cells which are dried on an infrared transparent matrix and scanned at the frequency range from 3000-950 cm.sup.-1. The identification of samples is based on establishing a calibration using a representative set of spectra of normal, dysplastic and malignant specimens. During the calibration process, multivariate techniques such as Principal Component Analysis (PCA) and/or Partial Least Squares (PLS) are used. PCA and PLS reduce the data based on maximum variations between the spectra, and generate clusters in a multidimensional space representing the different populations. The utilization of Mahalinobis distances, or linear regression (e.g., Principle Component Regression on the reduced data from PCA) form the basis for the discrimination. This method is simple to use and achieves statistically reliable distinction between the following groups of cervical smears: normal (individuals with no prior history of dysplasia), dysplasia and malignant samples. Lastly, this invention discloses a method to obtain the IR spectrum of individual cervical cells fixed on an infrared transparent matrix.