The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 12, 1999
Filed:
Nov. 14, 1994
Hiroki Kaneko, Tokyo, JP;
Toshikazu Takada, Tokyo, JP;
Jiro Shimada, Tokyo, JP;
Takashi Kuriki, Osaka, JP;
Michiyo Yanase, Osaka, JP;
Hiroki Takata, Osaka, JP;
Shigetaka Okada, Osaka, JP;
NEC Corporation, Tokyo, JP;
Ezaki Glico Kabushiki Kaisha, Osaka, JP;
Abstract
An enzyme is altered by introducing an additional hydrophobic group to enhance the hydrophobic environment in the enzyme so as to interfere with entrance of a water molecule. In this connection, the site of introduction and kind of hydrophobic group to be introduced are selected on the basis of comprehensive analysis of amino acid sequence, three-dimensional structure, reaction mechanism or the like of the target enzyme. Using this method, an amino acid residue of neopullulanase derived from Bacillus stearothermophilus was replaced by another amino acid.