The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 03, 1998
Filed:
Oct. 23, 1991
Applicant:
Inventors:
Ramnath Sasisekharan, Arlington, MA (US);
Kelley Moremen, Athens, GA (US);
Charles L Cooney, Brookline, MA (US);
Joseph J Zimmermann, Elm Grove, WI (US);
Robert S Langer, Newton, MA (US);
Assignee:
Massachusetts Institute of Technology, Cambridge, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
4352523 ; 435-2 ; 435 691 ; 435 698 ; 435 712 ; 4351723 ; 435232 ; 4353201 ; 935 10 ; 935 14 ; 935 27 ; 935 56 ; 935 72 ;
Abstract
The cloning of the heparinase gene from Flavobacterium Heparinum using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino acid sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. coli.