The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 11, 1997
Filed:
May. 19, 1995
David R Sibley, Rockville, MD (US);
Frederick J Monsma, Baltimore, MD (US);
Lawrence C Mahan, Bethesda, MD (US);
Loris D McVittie, Bethesda, MD (US);
Abstract
The present invention relates to the molecular cloning and expression of the D.sub.1 dopamine receptor protein that is linked to the activation of adenylyl cyclase activity. By constructing cell lines that express the D.sub.1 receptor, the affinities and efficacies of agonist and antagonist drugs with the receptor can be assessed. The present invention further relates to a recombinant DNA construct that includes a vector and a DNA fragment encoding the D.sub.1 receptor. The present invention also relates to a host cell transformed with a recombinant DNA construct, so that the DNA fragment is expressed and the D.sub.1 receptor is produced. Suitable expression systems include eukaryotic and procaryotic cells, especially mammalian cells such as rat or human. The present invention further relates to the antibody to the D.sub.1 receptor protein. For diagnostic purposes, antibodies to this receptor can be prepared by producing all or a portion of the receptor protein and injecting these into various types of mammals. Using the resulting antibodies, expression of the D.sub.1 receptor cDNA in cells can be measured.