The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 29, 1996
Filed:
Jan. 26, 1995
Applicant:
Inventors:
Ramnath Sasisekharan, Arlington, MA (US);
Daniel L Lohse, Bryan, TX (US);
Charles L Cooney, Brookline, MA (US);
Robert J Linhardt, Iowa City, IA (US);
Robert S Langer, Newton, MA (US);
Assignees:
Massachusetts Institute of Technology, Iowa City, IA (US);
University of Iowa Research Foundation, Cambridge, MA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435220 ; 435100 ; 435183 ; 435232 ; 435822 ; 435850 ;
Abstract
A single, reproducible scheme to simultaneously purify all three of the heparin lyases from F. heparinum to apparent homogeneity is disclosed herein. The kinetic properties of the heparin lyases have been determined as well as the conditions to optimize their activity and stability. Monoclonal antibodies to the three heparinases are also described and are useful for detection, isolation and characterization of the heparinases.