The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 28, 1992
Filed:
Jun. 27, 1990
Elli Rauscher, Munich, DE;
Ulrich Neumann, Peissenberg, DE;
August W Wahlefeld, Weilheim, DE;
Alexander Hagen, Tutzing, DE;
Wolfgang Gruber, Tutzing-Unterzeismering, DE;
Joachim Ziegenhorn, Unterpfaffenhofen, DE;
Eugen Schaich, Weilheim, DE;
Ulfert Deneke, Peissenberg, DE;
Michal Gerhard, Tutzing, DE;
Gunter Weimann, Tutzing, DE;
Boehringer Mannheim GmbH, Mannheim, DE;
Abstract
.alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided. A process for the preparation of a maltoheptaose will Bacillus macerans amylase, free of p-nitrophenyl-alpha-glucoside splitting activity is disclosed.