The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 11, 1990

Filed:

Jun. 24, 1987
Applicant:
Inventors:

Atte J von Wright, Espoo, FI;

Seppo K Sivela, Helsinki, FI;

Soile S Tynkkynen, Espoo, FI;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12P / ;
U.S. Cl.
CPC ...
4352523 ; 435 691 ; 435 711 ; 435 712 ; 4351723 ; 43525232 ; 43525233 ; 43525235 ; 435320 ; 935 29 ; 935 72 ; 935 73 ; 935 74 ; 935 75 ;
Abstract

The invention relates to a cloning vector suited for the souring bacteria of dairy industries, which cloning vector is based on a 1.7 kb ClaI fragment of the Streptococcus lactis cryptic plasmid pSH71. Therein are inserted a chloramphenicol resistance gene from the Streptococcus sanguis plasmid pGB301 and an erythromycin resistance gene from the Staphylococcus aureus plasmid pE194, a 0.35 kb fragment from the Escherichia coli plasmis pBR322 being inserted therebetween. The size of the cloning vector is 5.0 kb. The cloning vector enables the use of 'forced cloning' methods. It possesses a wide host spectrum (group N streptococci, Escherichia coli, Bacillus subtilis), and can be applied for improving the souring properties of the group N streptococci and for the preparation of foreign proteins in these bacteria or other hosts.


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