The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 18, 2025

Filed:

Jul. 14, 2023
Applicant:

Codexis, Inc., Redwood City, CA (US);

Inventors:

Jeffrey C. Moore, Westfield, NJ (US);

Jack Liang, South San Francisco, CA (US);

Jonathan Penfield, Truckee, CA (US);

Jovana Nazor, Milpitas, CA (US);

Nikki Dellas, San Carlos, CA (US);

Vesna Mitchell, Santa Clara, CA (US);

Da Duan, Newark, CA (US);

Iman Farasat, Rahway, NJ (US);

Agustina Rodriguez-Granillo, Rahway, NJ (US);

Grant Murphy, Rahway, NJ (US);

Nicholas Marshall, Rahway, NJ (US);

Assignee:

Codexis, Inc., Redwood City, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 41/00 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 15/70 (2006.01);
U.S. Cl.
CPC ...
C12P 41/002 (2013.01); C12N 9/0004 (2013.01); C12N 9/0006 (2013.01); C12N 15/70 (2013.01); C12Y 101/01002 (2013.01); C12Y 120/01001 (2013.01);
Abstract

The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketoreductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase enzymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system.


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