The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 21, 2025

Filed:

Jun. 18, 2018
Applicant:

River Stone Biotech, Inc., Wilmington, DE (US);

Inventors:

Markus Schwab, Reinach, CH;

Franziska Grassinger, Reinach, CH;

Laura Occhipinti, Reinach, CH;

Philipp Friedrich Berninger, Reinach, CH;

Jon Richard Heal, Reinach, CH;

Joseph Michael Sheridan, Reinach, CH;

Anaelle Hatsch, Reinach, CH;

Jens Houghton-Larsen, Copenhagen, DK;

Assignee:

RIVER STONE BIOTECH, INC., Wilmington, DE (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 17/16 (2006.01); C12N 1/21 (2006.01); C12N 9/88 (2006.01); C12N 15/60 (2006.01);
U.S. Cl.
CPC ...
C12P 17/165 (2013.01); C12N 9/88 (2013.01); C12Y 402/01078 (2013.01);
Abstract

The invention relates to norcoclaurine synthases and substrate binding sites having one or more site-specific mutation which increase the activity, when compared to the wild type synthase, of the condensation of 4-HPAA and dopamine to (S)-norcoclaurine and/or 3,4-DhPAA and dopamine to (S)-norlaudanosoline. The inventors both identified specific mutations corresponding to at position 73, 75, 77, 82, 99, 114, 141, 142, 147, 152, 174 and/or 178 in the count according to SEQ ID No: 1, and sites corresponding to the binding domains defined in SEQ ID NO: 4 and 5, where the mutated increase of the activity may be positioned within these norcoclaurine synthases. These domains are conserved regions.


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