The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 01, 2024
Filed:
Aug. 31, 2022
Zhejiang University of Technology, Zhejiang, CN;
Yaping Xue, Zhejiang, CN;
Shuping Zou, Zhejiang, CN;
Zhentao Jiang, Zhejiang, CN;
Jianmiao Xu, Zhejiang, CN;
Feng Cheng, Zhejiang, CN;
Yuguo Zheng, Zhejiang, CN;
ZHEJIANG UNIVERSITY OF TECHNOLOGY, Zhejiang, CN;
Abstract
A cofactor self-sufficientand its construction method and application in the synthesis of L-glufosinate are provided. The present invention expresses a NADH kinase and key enzymes of the cofactor synthesis pathway in, and knocks out the genes of enzymes that catabolizes cofactor, and with the addition of co-metabolic intermediates during cell incubation, the intracellular NADP(H) concentration is increased by at least 50% and the catalytic activity of glufosinate dehydrogenase by 2-fold, resulting in a significant increase in the spatiotemporal yield of the glufosinate synthesis reaction.