The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 01, 2024
Filed:
Aug. 11, 2021
Jiangnan University, Wuxi, CN;
Zhemin Zhou, Wuxi, CN;
Zhongmei Liu, Wuxi, CN;
Ting Li, Wuxi, CN;
Dezhi Chen, Wuxi, CN;
Sailan Zhang, Wuxi, CN;
Li Zhou, Wuxi, CN;
Wenjing Cui, Wuxi, CN;
Zhongyi Cheng, Wuxi, CN;
Junling Guo, Wuxi, CN;
JIANGNAN UNIVERSITY, Wuxi, CN;
Abstract
The disclosure discloses a mutant of nitrile hydratase derived from, and belongs to the technical field of enzyme engineering. The nitrile hydratase mutantNhase-A20V provided by the disclosure has a half-life of about 10 min at 70° C., which does not change much compared with the thermal stability of the wild enzyme. The specific enzyme activity of the mutantNhase-A20V is 128% of that of the wild enzyme. At the same time, the mutant also has better tolerance to a substrate and a product, and the final yield of nicotinamide produced by whole-cell catalysis reaches 598 g/L. Therefore, the nitrile hydratase mutantNhase-A20V provided by the disclosure has good enzymatic properties and is beneficial to future industrial production.