The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 19, 2024
Filed:
Jan. 03, 2022
Shan Dong University, Jinan, CN;
Bloomage Biotechnology Corporation Limited, Jinan, CN;
Juzheng Sheng, Jinan, CN;
Xueping Guo, Jinan, CN;
Jianqun Deng, Jinan, CN;
Fengshan Wang, Jinan, CN;
Zhen Lu, Jinan, CN;
Ranran Du, Jinan, CN;
Liu Sun, Jinan, CN;
Yuanjun Sun, Jinan, CN;
SHAN DONG UNIVERSITY, Jinan, CN;
Bloomage Biotechnology Corporation Limited, Jinan, CN;
Abstract
A heparin skeleton synthase originates from, with an amino acid sequence as shown in SEQ ID NO.2 and a nucleotide sequence of the coding gene as shown in SEQ ID NO.1. Its recombinant expression level is 6.8 times that of the existing heparin skeleton synthase KfiA fromK5, and total enzyme activity per fermentation liquor is 5.22 times that of the heparin skeleton synthase KfiA. The heparin skeleton synthase mutants obtained through site-directed mutagenesis of the sites No. 16, No. 25, No. 30, No. 111, No. 165, and No. 172 in the amino acid sequence of the said heparin skeleton synthase all have high expression levels.