The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 31, 2023
Filed:
Oct. 30, 2019
Tianjin University of Science & Technology, Tianjin, CN;
Xixian Xie, Tianjin, CN;
Heyun Wu, Tianjin, CN;
Jiachu Wang, Tianjin, CN;
Faqing Wu, Tianjin, CN;
Xiaoqian Liu, Tianjin, CN;
Yanan Hao, Tianjin, CN;
TIANJIN UNIVERSITY OF SCIENCE & TECHNOLOGY, Tianjin, CN;
Abstract
A genetically engineered strain having high-yield of L-valine is disclosed. Starting fromW3110, an acetolactate synthase gene alsS ofis inserted into a genome thereof and overexpressed; a ppGpp 3'-pyrophosphate hydrolase mutant R290E/K292D gene spoTM ofis inserted into the genome and overexpressed; a lactate dehydrogenase gene ldhA, a pyruvate formate lyase I gene pflB, and genes frdA, frdB, frdC, frdD of four subunits of fumaric acid reductase are deleted from the genome; a leucine dehydrogenase gene bcd ofreplaces a branched chain amino acid transaminase gene ilvE of; and an acetohydroxy acid isomeroreductase mutant L67E/R68F/K75E gene ilvCM replaces the native acetohydroxy acid isomeroreductase gene ilvC of. Furthermore, the L-valine fermentation method is improved by using a two-stage dissolved oxygen control. The L-valine titer and the sugar-acid conversion rate are increased.