The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 06, 2022
Filed:
Dec. 24, 2018
Jiangsu Guoxin Union Energy Co., Ltd, Jiangsu, CN;
Jiangnan University, Jingsu, CN;
Guiyang Shi, Jiangsu, CN;
Zhijie Hu, Jiangsu, CN;
Youran Li, Jiangsu, CN;
Xiaodong Jiang, Jiangsu, CN;
Sai Jin, Jiangsu, CN;
Fuxin Sun, Jiangsu, CN;
Cheng Zhang, Jiangsu, CN;
Dongjiao Zhou, Jiangsu, CN;
Jiawei Lu, Jiangsu, CN;
Maodong Miao, Jiangsu, CN;
Zihao Fan, Jiangsu, CN;
Jiangsu Guoxin Union Energy Co., Ltd, Jiangsu, CN;
JIANGNAN UNIVERSITY, Jiangsu, CN;
Abstract
Disclosed is anseed continuous culture method, comprising the steps of: (1) at a startup stage,spores are inoculated into a seed culture medium to obtain a seed liquid; (2) at a seed continuous culture stage, continuous dispersion treatment is performed on the seed liquid obtained in step (1), continuous culture is performed on the seed liquid obtained by dispersion, and meanwhile, a fresh seed feed medium is replenished; and (3) at a stop stage, the replenishment of the fresh seed feed medium and the dispersion treatment are stopped, continuous culture is performed to obtain a seed liquid, and then the seed liquid is transferred into the fermentation medium for fermentation culture. The method according to the present invention makes breakthrough to solve problems that multi-cellular filamentous bacteria grow slowly and mycelium pellets are easy to lose in continuous culture, thus fully achieving seed continuous culture.