The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 28, 2021
Filed:
Oct. 31, 2018
The Regents of the University of California, Oakland, CA (US);
Erkin Seker, Davis, CA (US);
Joshua Hihath, Woodland, CA (US);
Maria Marco, Davis, CA (US);
Paul Feldstein, Sacramento, CA (US);
Pallavi Daggumati, Emeryville, CA (US);
Yuanhui Li, Davis, CA (US);
Zimple Matharu, Davis, CA (US);
Juan Artes Vivancos, Amstelveen, NL;
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US);
Abstract
A biosensor platform apparatus and method are provided that can detect, purify and identify nucleic acid (DNA and RNA) biomarkers in complex biological fluids. The methods use a two-stage molecular based approach. The first stage screens for specific nucleic acid-based biomarkers in complex biological fluids by electrochemical detection of DNA:RNA hybridization and facilitates the removal of remaining complex media constituents. The first stage utilizes probes within a tunable nanoporous electrode. The second stage identifies the purified specific hybrids by single-molecule conductance measurements via break junction scanning. Identification can be assisted with a library of conductance measurements. The methods can provide strain level information that can be used for identifying anti-microbial resistance in detected pathogens. Collection of RNA targets allows for biomarker detection and identification without the need for amplification and can provide information about the viability of the sample organism.