The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 08, 2021

Filed:

Jan. 26, 2017
Applicant:

Just-evotec Biologics, Inc., Seattle, WA (US);

Inventors:

Jeffrey T. McGrew, Woodinville, WA (US);

Pauline S. Smidt, Seattle, WA (US);

E-Ching Ong, Seattle, WA (US);

Assignee:

JUST-EVOTEC BIOLOGICS, INC., Seattle, WA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 15/86 (2006.01); C12N 15/85 (2006.01); C12N 9/38 (2006.01);
U.S. Cl.
CPC ...
C12N 15/86 (2013.01); C12N 9/2471 (2013.01); C12N 15/85 (2013.01); C12Y 302/01023 (2013.01); C12N 2710/16122 (2013.01); C12N 2710/16143 (2013.01); C12N 2830/003 (2013.01); C12N 2830/15 (2013.01); C12N 2830/42 (2013.01); C12N 2840/105 (2013.01);
Abstract

A hybrid promoter for recombinant expression of proteins of interest is disclosed that combines a mCMV enhancer sequence with a rat EF-1alpha intron sequence. Also disclosed are an expression cassette containing the hybrid promoter and a recombinant expression vector containing the expression cassette. A mammalian host cell, which comprises the recombinant expression vector is also disclosed, as is a method of producing a protein of interest that employs the mammalian host cell, optionally involving tetracycline-inducible expression of the protein.


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