The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 24, 2020
Filed:
Jun. 27, 2014
Applicant:
New England Biolabs, Inc., Ipswich, MA (US);
Inventors:
Zhenyu Zhu, Beverly, MA (US);
Janine Borgaro, Beverly, MA (US);
Aine Quimby, Newton, NH (US);
Shengxi Guan, Stoneham, MA (US);
Zhiyi Sun, Gloucester, MA (US);
Assignee:
New England Biolabs, Inc., Ipswich, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12N 9/22 (2006.01); C12Q 1/34 (2006.01); C12Q 1/683 (2018.01);
U.S. Cl.
CPC ...
C12N 9/22 (2013.01); C12Q 1/683 (2013.01);
Abstract
Provided herein in some embodiments is a non-naturally occurring variant of a wild type restriction enzyme defined by SEQ ID NO: 20, wherein the variant has at least a 2 fold increase in cleavage at 5-β glucosylhydroxymethylcytosine (5βghmC) compared with methylcytosine relative to the wild type enzyme. Methods for examining hydroxymethylation of a DNA sample using the variant enzyme are also provided.