The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 16, 2019
Filed:
Feb. 09, 2018
New England Biolabs, Inc., Ipswich, MA (US);
Romualdas Vaisvila, Ipswich, MA (US);
Theodore B. Davis, Boxford, MA (US);
Shengxi Guan, Ipswich, MA (US);
Zhiyi Sun, Gloucester, MA (US);
Laurence Ettwiller, Ipswich, MA (US);
Lana Saleh, Hamilton, MA (US);
New England Biolabs, Inc., Ipswich, MA (US);
Abstract
Methods and compositions are provided for identifying any of the presence, location and phasing of methylated and/or hydroxymethylated cytosines in nucleic acids including long stretches of DNA. In some embodiments, the method may comprise reacting a first portion (aliquot) of a nucleic acid sample with a dioxygenase and optionally a glucosyltransferase in a reaction mixture containing the nucleic acid followed by a reaction with a cytidine deaminase to detect and optionally mapC in a DNA. Optionally, a second portion can be reacted with glucosyltransferase followed by reaction with a cytidine deaminase to detect and optionally mapC in a DNA.