The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 26, 2019

Filed:

Mar. 10, 2016
Applicants:

B Food Science Co., Ltd., Chita-shi, JP;

Microbiopharm Japan Co., Ltd., Chuo-ku, JP;

Inventors:

Takumi Tochio, Chita, JP;

Naomi Ito, Chita, JP;

Saki Nakamura, Chita, JP;

Yoshimi Fukatani, Chita, JP;

Tadashi Fujii, Chuo-ku, JP;

Keisuke Tamura, Chuo-ku, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/26 (2006.01); C12P 19/14 (2006.01); C12P 21/02 (2006.01); C12N 15/09 (2006.01);
U.S. Cl.
CPC ...
C12N 9/2431 (2013.01); C12N 15/09 (2013.01); C12P 19/14 (2013.01); C12P 21/02 (2013.01); C12Y 302/01026 (2013.01);
Abstract

To provide an improved β-fructofuranosidase which is capable of efficiently producing kestose while inhibiting the production of nystose. This improved β-fructofuranosidase comprises either: an amino acid sequence (a) obtained by introducing, into the amino acid sequence represented by SEQ ID NO: 2, an amino acid mutation i) in which the 85th glycine (G) from the N-terminal is substituted for a protein-constituting amino acid other than glycine (G), and/or an amino acid mutation ii) in which the 310th histidine (H) from the N-terminal is substituted for lysine (K), arginine (R), or tyrosine (Y); or an amino acid sequence (b) which exhibits β-fructofuranosidase activity, and which comprises an amino acid sequence obtained by deleting, substituting, inserting, or adding one or more amino acids in (a) other than the amino acid into which the amino acid mutation has been introduced.


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