Wolfgang Stiege

Title: Innovations and Inventions by Wolfgang Stiege

Introduction

Wolfgang Stiege, an accomplished inventor based in Berlin, Germany, has made noteworthy contributions to the field of biotechnology with his innovative methodologies. Holding two patents, he has played a significant role in advancing techniques for protein and peptide production as well as gene expression.

Latest Patents

Stiege's latest inventions include a method for the production of proteins and peptides, which involves several critical steps. This method utilizes a cell-free lysate derived from eukaryotic cells, which contains functional microsomal vesicles. By introducing a nucleic acid coding for the desired protein or peptide along with a signal sequence, the lysate is maintained at a temperature between 20 to 35°C, allowing for the translocation of the formed proteins or peptides into the microsomal vesicles. Afterward, the microsomal vesicles are dissolved and the resultant proteins or peptides can be separated from the lysate as needed.

In addition, his second patent details a method for gene expression in a cell-free transcription/translation system. This innovative approach encompasses all necessary components for the transcription and translation mechanisms, including amino acids, nucleotides, and metabolic components that provide energy for synthesis. Remarkably, the proteins produced during translation are then immobilized on a matrix, enhancing the efficiency of the process.

Career Highlights

Wolfgang Stiege is associated with Rina Netzwerk RNA Technologien GmbH, a firm known for its cutting-edge research in RNA technologies. His efforts and inventions have significantly contributed to the research and development landscape within the biotechnology sector.

Collaborations

Throughout his career, Stiege has collaborated with notable colleagues, including Volker A Erdmann and Thorsten Lamla. These partnerships have fostered an environment of innovation and progress, further amplifying the impact of his inventions within the scientific community.

Conclusion

Wolfgang Stiege's innovative methods for protein and peptide production, along with his gene expression techniques, underscore his commitment to advancing biotechnological processes. His contributions as an inventor continue to influence research and application in the field, while his collaborations enhance the collective knowledge and capabilities of his field.