William Harold Donish

Title: The Innovative Contributions of William Harold Donish

Introduction

William Harold Donish is a notable inventor based in Rochester, NY (US), recognized for his significant contributions to the field of molecular diagnostics. With a total of 6 patents to his name, Donish has made strides in enhancing the efficiency and safety of nucleic acid amplification and detection technologies.

Latest Patents

Among his latest innovations is the "Containment cuvette for PCR and method of use." This invention is designed to prevent nucleic acid amplified by PCR technology from being released into the atmosphere while allowing for detection steps to ascertain the presence of nucleic acid. The cuvette features compartments that can either have detection reagents pre-incorporated or added post-amplification, with a check valve to prevent the release of amplified nucleic acid. The transfer of liquids between compartments is facilitated by flexible compartment walls and an external pressure source or pistons integrated into the cuvette.

Another significant patent is the "Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle." This method allows for the amplification and detection of nucleic acids using a rapid polymerase chain reaction procedure, which includes critically defined temperature and time parameters. Each cycle of this process typically takes less than two minutes, with some cases requiring less than 90 seconds. The method employs at least 5 units/100 µl of thermostable DNA polymerase and optimized primer concentrations to enable quick cycling.

Career Highlights

William Harold Donish has had a distinguished career, working with prominent companies such as Johnson & Johnson Clinical Diagnostics, Inc. and Eastman Kodak Company. His experience in these organizations has contributed to his expertise in the field of diagnostics and molecular biology.

Collaborations

Throughout his career, Donish has collaborated with notable individuals, including John Bruce Findlay and Paul Nicholas Schnipelsky. These partnerships have likely enriched his work and led to further advancements in his inventions.

Conclusion

William Harold Donish's innovative work in the field of molecular diagnostics has made a lasting impact, particularly through his patents that enhance nucleic acid amplification and detection methods. His contributions continue to influence the industry and pave the way for future advancements.