William E Keene

Title: William E. Keene: Innovator in Serodiagnostic Reagents

Introduction

William E. Keene is a notable inventor based in Berkeley, California. He has made significant contributions to the field of serodiagnostic reagents, particularly in relation to Entamoeba histolytica. His work has implications for diagnosing infections caused by this pathogen.

Latest Patents

William E. Keene holds a patent for a serodiagnostic reagent for Entamoeba histolytica. The invention is a composition of matter that consists of a purified homogeneous thiol proteinase secreted by Entamoeba histolytica trophozoites. This neutral thiol proteinase has a subunit molecular weight of approximately 56,000 ± 4,000, a neutral pH optimum, and an isoelectric point of about 6. The enzyme can be purified using anion exchange and chromatofocusing Fast Protein Liquid Chromatography (FPLC). The resulting homogeneous neutral thiol proteinase is immunogenic and can be utilized in ELISA assays or other standard immunoassays to identify antibodies in individuals exposed to Entamoeba histolytica trophozoites.

Career Highlights

William E. Keene is affiliated with the University of California, where he has conducted research and developed his innovative patent. His work has contributed to advancements in diagnostic methods for infectious diseases.

Collaborations

William E. Keene has collaborated with James H. McKerrow, enhancing the research and development of serodiagnostic reagents.

Conclusion

William E. Keene's contributions to the field of serodiagnostic reagents highlight the importance of innovation in medical diagnostics. His work continues to impact the identification and treatment of infections caused by Entamoeba histolytica.