Company Filing History:
Years Active: 2024-2025
Title: Innovations of Wanping Chen in DNA Synthesis
Introduction
Wanping Chen is a prominent inventor based in Wuhan, China. He has made significant contributions to the field of biotechnology, particularly in the synthesis and assembly of DNA. With a total of three patents to his name, Chen's work is paving the way for advancements in genetic engineering.
Latest Patents
Chen's latest patents include an efficient synthesis and assembly method for large fragment DNA based on programmable nuclease Argonaute. This method involves constructing and treating antibiotic resistance gene reconstructed vectors with linearization. It also includes dividing a target DNA into multiple small DNA fragments, synthesizing these fragments, and loading them onto the antibiotic resistance gene reconstructed vectors. The method combines the SLIC technique with a resistance gene reconstruction strategy, allowing for the assembly of 5-6 small fragments in a single reconstruction, which is more efficient and time-saving. Additionally, mutations caused by PCR are avoided, and the reconstructed large fragment does not require a second sequencing, thus saving time and costs.
Another notable patent is related to the PfAgo mutant protein, which exhibits mesophilic target nucleic acid cleavage activity. This mutant protein has specific amino acid mutations that enhance its activity within a temperature range of 30-95 degrees Celsius. This innovation effectively expands the application range of prokaryotic Argonaute (pAgo) proteins, making it a valuable tool in genetic research.
Career Highlights
Wanping Chen is affiliated with Hubei University, where he continues to engage in groundbreaking research. His work has garnered attention in the scientific community, contributing to advancements in DNA synthesis and genetic engineering.
Collaborations
Chen collaborates with notable colleagues, including Lixin Ma and Fei Wang, who share his passion for innovation in biotechnology. Their combined efforts are instrumental in pushing the boundaries of current scientific understanding.
Conclusion
Wanping Chen's contributions to the field of DNA synthesis and assembly are noteworthy. His innovative patents reflect a commitment to advancing biotechnology and enhancing genetic research methodologies.