Wanlin Liu

Title: Wanlin Liu: Innovator in Transcription Factor Research

Introduction

Wanlin Liu is a prominent inventor based in Beijing, China. He has made significant contributions to the field of molecular biology, particularly in the study of transcription factors. His innovative work has led to the development of a unique method for enriching and isolating endogenous transcription factors and their complexes.

Latest Patents

Wanlin Liu holds a patent titled "Method for enrichment and isolation of endogenous transcription factor and complexes thereof and corresponding tandem arrays of concatenated transcription factor response elements." This invention provides a method for the enrichment and isolation of endogenous transcription factors and their complexes. The method utilizes the property of transcription factors binding to sequence-specific DNA elements during the regulation of gene expression. The corresponding tandem arrays of concatenated transcription factor response elements (catTFRE) are designed and synthesized as dual copies of DNA response elements for various transcription factors. The synthesized catTFRE is cloned into a target vector, and biotinylated catTFRE with 200 bp arms is prepared using a PCR strategy. For the enrichment and isolation of endogenous transcription factors and their complexes, the biotinylated catTFRE is immobilized to streptavidin-coated magnetic beads and incubated with nuclear extract. This process allows for the isolation of endogenous transcription factors and their complexes from the nuclear extract, with further identification possible through mass spectrometry or other functional characterization methods.

Career Highlights

Throughout his career, Wanlin Liu has worked with esteemed institutions such as Baylor College of Medicine and the Institute of Radiation Medicine, China Academy of Military Medical Sciences. His research has significantly advanced the understanding of gene regulation and transcription factor dynamics.

Collaborations

Wanlin Liu has collaborated with notable colleagues, including Jun Qin and Chen Ding. Their collective efforts have contributed to the advancement of research in the field of transcription factors.

Conclusion

Wanlin Liu's innovative methods and research in transcription factor isolation have made a lasting impact on molecular biology. His contributions continue to influence the understanding of gene expression regulation.