Company Filing History:
Years Active: 2002-2003
Title: Tomas Frgala: Innovating in Viable Cell Quantification
Introduction
Tomas Frgala is an inventive mind based in Brno, Czech Republic, recognized for his significant contributions to the field of microscopy and cell analysis. His innovative approach has led to the development of patented methods that enhance the understanding and measurement of viable cells.
Latest Patents
Tomas has been granted two patents, with his latest being a pioneering method for preparing cell samples for the quantification of viable cells using a fluorescence digital imaging microscopy system. This invention employs a digital thresholding technique where cell samples are stained with a first fluorescent dye, which accumulates in viable cells, while a second quenching dye is used that enters only non-viable cells. This setup significantly reduces background fluorescence, thereby improving the dynamic range and accuracy of viable cell number measurements. For instance, in low viability cultures treated with fluorescein diacetate, background fluorescence often masked viable cells. However, through the application of digital thresholding and eosin treatment, background fluorescence was effectively reduced, allowing for a linear response over four logs of viable cell density.
Career Highlights
Currently, Tomas Frgala is associated with Children's Hospital Los Angeles, where he continues to push the boundaries of medical imaging technology and cell biology. His work is critical in providing more accurate assessments of cell viability, which is essential in various research and clinical applications.
Collaborations
Tomas collaborates closely with esteemed coworker C. Patrick Reynolds, contributing to advancements in their respective fields. Their joint efforts focus on merging innovative techniques with practical applications in healthcare, underscoring the importance of teamwork in scientific research.
Conclusion
Tomas Frgala exemplifies the spirit of innovation through his dedication to improving viable cell quantification methods. His contributions not only advance scientific knowledge but also pave the way for better healthcare solutions, demonstrating the impactful intersection of technology and medicine. The patents developed by Tomas reflect his commitment to excellence and the ongoing evolution of research methodologies in the life sciences.