This inventor holds 3 USPTO granted patents. Top assignees: Eastman-Kodak Company, Johnson & Johnson Clinical Diagnostics, Inc.. Active years: 1993-1996.
Company Filing History:
2 out of 21,604 
1 out of 92 
Years Active: 1993-1996
Title: Innovations by Tobias D Ekeze
Introduction
Tobias D Ekeze is an accomplished inventor based in Rochester, NY. He holds a total of 3 patents that showcase his expertise in the field of nucleic acid extraction. His innovative methods have significant implications for genetic research and diagnostics.
Latest Patents
One of his notable inventions is a method for extracting deoxyribonucleic acids without using proteolytic enzymes. This invention provides a rapid and highly effective way to extract nucleic acids from cells or virions. The extraction process is completed within minutes using a lysing composition that includes a buffer, a source of a DNA polymerase cofactor, a stabilizer, and at least one nonionic surfactant. This composition effectively releases nucleic acids from the cytoplasmic and nuclear membranes of cells or virions. The resulting mixture is then heated to boiling for up to fifteen minutes, allowing for the recovery of nucleic acids for amplification using polymerase chain reaction.
Another significant patent involves methods of extracting, amplifying, and detecting nucleic acids from whole blood or a peripheral blood mononuclear cell (PBMC) fraction. This rapid extraction method is particularly useful for detecting genetic diseases or infectious agents, such as HIV-I. The PBMC fraction is heated at or near the boiling point of water for a few minutes, and the extracted nucleic acid is recovered for further analysis. This method can also be applied to whole blood mixed with a salt solution containing a polysaccharide, such as dextran.
Career Highlights
Tobias D Ekeze has worked with prominent companies, including Eastman Kodak Company and Johnson & Johnson Clinical Diagnostics, Inc. His experience in these organizations has contributed to his development as an inventor and innovator in the field of biotechnology.
Collaborations
Throughout his career, Ekeze has collaborated with notable colleagues such as Thomas J Cummins and Brent A Burdick. These collaborations have likely enriched his work and contributed to the success of his inventions.
Conclusion
Tobias D Ekeze is a significant figure in the field of nucleic acid extraction, with innovative patents that enhance genetic research and diagnostics. His contributions continue to impact the scientific community positively.