Company Filing History:
Years Active: 2000-2001
Title: Innovations by Timothy W Nilsen
Introduction
Timothy W Nilsen is an accomplished inventor based in Russell, OH (US). He holds 2 patents that focus on innovative methodologies in the field of genetics and molecular biology. His work has significant implications for the identification and manipulation of essential genes.
Latest Patents
Nilsen's latest patents include a method for identifying essential or functional genes. This invention provides two methodologies: the first allows for the rapid identification of essential genes, while the second facilitates the acquisition of biologically active nucleic molecules, such as ribozymes and antisense RNA. In the first method, a library of external guide sequences (EGSs) is prepared to target bacterial RNAse for cleaving substrates. Cells containing the genes to be screened are treated with these EGSs, and those that result in a loss of viability are analyzed to identify the responsible gene. The second method focuses on identifying nucleotide molecules with optimal biological activity, enabling the cleavage of a gene of interest by RNase P through a vector that includes two reporter genes.
Another notable patent involves a random intracellular method for obtaining optimally active nucleic acids. This method identifies affector RNA molecules that inhibit the expression of target RNA molecules. By screening a library of potential affector RNA molecules, Nilsen's method allows for the identification of those that effectively inhibit expression in an in vivo setting. This innovative approach provides a comprehensive assessment of accessible sites in the RNA of interest in a single assay.
Career Highlights
Throughout his career, Timothy W Nilsen has made significant contributions to the field of molecular biology. He has worked with notable organizations, including Innovir Laboratories, Inc. and Yale University. His expertise and innovative methodologies have positioned him as a key figure in genetic research.
Collaborations
Nilsen has collaborated with various professionals in his field, including Thomas J Kindt, enhancing the impact of his research and inventions.
Conclusion
Timothy W Nilsen's contributions to the field of genetics through his innovative patents demonstrate his commitment to advancing scientific knowledge. His methodologies for identifying essential genes and developing biologically active nucleic molecules have the potential to revolutionize genetic research and applications.