Thor Lns Holtet

Title: Innovations of Thor Lns Holtet in Protein Refolding

Introduction

Thor Lns Holtet is a notable inventor based in Aarhus V, Denmark. He has made significant contributions to the field of protein refolding, particularly through his innovative methods that enhance the efficiency of producing correctly folded proteins. His work is crucial for various applications in biotechnology and pharmaceuticals.

Latest Patents

Thor Lns Holtet holds a patent for an "Interactive method of at least three cycles for the refolding of proteins." This novel method is designed to produce correctly folded proteins from a mixture of misfolded proteins, such as bacterial inclusion-body aggregates. A major aspect of this method is the achievement of overall efficiency through a time-sequence of multiple denaturation-renaturation cycles. This results in the gradual accumulation of correctly folded proteins. The method has proven effective for a variety of recombinant proteins. Additionally, it includes novel encrypted recognition sites for bovine coagulation factor X.sub.a, which can be activated in vitro by controlled oxidation or reversible derivatization of cysteine residues, generating new cleavage sites for factor X.sub.a. Furthermore, two new recombinant serine proteases exhibiting narrow substrate specificity for factor X.sub.a recognition sites are also part of his patent, which may replace natural coagulation factor X.sub.a for the cleavage of chimeric proteins.

Career Highlights

Thor Lns Holtet is associated with Denzyme Aps, where he continues to develop and refine his innovative methods. His work has garnered attention in the scientific community for its potential applications in improving protein production processes.

Collaborations

Thor has collaborated with notable colleagues, including Hans Christian Tho Slashedgersen and Michael Etzerodt. These collaborations have likely contributed to the advancement of his research and the development of his patented methods.

Conclusion

Thor Lns Holtet's innovative approach to protein refolding represents a significant advancement in biotechnology. His contributions are essential for enhancing the efficiency of protein production, which has far-reaching implications in various scientific fields.