Takeharu Masaki

Title: The Innovations of Inventor Takeharu Masaki

Introduction

Takeharu Masaki is a notable inventor based in Ibaraki, Japan, credited with three patents that showcase his contributions to the field of biotechnology and enzymology. His work exemplifies a commitment to advancing scientific knowledge and practical applications in various industries.

Latest Patents

Among his most significant innovations is a novel alkaline protease that exhibits unique characteristics. This enzyme has a molecular weight of 30,000, determined through a gel filtration method using Sephadex G-75. It possesses an isoelectric point of 5.3 and shows optimum pH reactivity for esterase activity at pH 8.5 and amidase activity at pH 9.0. Notably, this protease selectively hydrolyzes the ester bond and the amide bond derived from the carboxyl group of L-lysine. Additionally, it is inhibited by diisopropyl phosphofluoride, tosyl-L-lysine chloro-methyl ketone, and phenylmethylsulfonyl chloride. The preferred microbial source for producing this protease is from the genus Achromobacter.

Another of Masaki's important patents involves the semi-synthesis of human insulin. This process relates to preparing B30-threonine-insulin by reacting des-B30-insulin with an excess of a threonine derivative. This reaction occurs in the presence of an enzyme that acts specifically on lysine carbonyl in peptide bondings and is produced by Achromobacter lyticus M 497-1.

Career Highlights

Takeharu Masaki has worked with prominent companies such as Shionogi & Company Limited and Wako Pure Chemical Industries, Inc. His career in these reputable organizations allowed him to significantly impact the advancing field of biochemistry.

Collaborations

Throughout his career, Masaki has collaborated with esteemed coworkers, including Masami Soejima and Kazuyuki Morihara. These professional relationships have likely fostered an environment for innovative thinking and experimentation, essential for successful research and development.

Conclusion

Takeharu Masaki's pioneering work in the development of novel enzymes and methods for insulin production marks him as an influential figure in the field of biotechnology. His dedication to innovation continues to pave the way for future advancements, reflecting the vital role inventors play in shaping scientific progress.