Location History:
- Berkeley, CA (US) (1987 - 1989)
- Albany, CA (US) (1996)
Company Filing History:
Years Active: 1987-1996
Title: The Innovations of Stephen J Scharf
Introduction
Stephen J Scharf is a notable inventor based in Berkeley, California. He has made significant contributions to the field of molecular biology, particularly in the area of DNA typing and nucleic acid amplification. With a total of three patents to his name, Scharf's work has had a lasting impact on genetic research and diagnostics.
Latest Patents
Scharf's latest patents include "Methods and reagents for HLA DRbeta DNA typing" and "Process for amplifying, detecting, and/or cloning nucleic acid sequences." The latter patent describes a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. This innovative process involves treating separate complementary strands of nucleic acid with a molar excess of two oligonucleotide primers. The primers are extended to form complementary primer extension products, which act as templates for synthesizing the desired nucleic acid sequence. The detection of the amplified sequence can be carried out stepwise or simultaneously, and the process can be repeated as often as desired. Additionally, a specific nucleic acid sequence may be cloned into a vector using primers that amplify the sequence, which contain restriction sites on their non-complementary ends.
Career Highlights
Throughout his career, Stephen J Scharf has worked with prominent companies in the biotechnology sector, including Cetus Corporation and Hoffmann-La Roche Inc. His experience in these organizations has allowed him to develop and refine his innovative techniques in molecular biology.
Collaborations
Scharf has collaborated with notable figures in the field, including Henry A Erlich and Kary B Mullis. These collaborations have further enhanced his research and contributed to the advancement of genetic technologies.
Conclusion
Stephen J Scharf's contributions to the field of molecular biology through his patents and collaborations have significantly advanced the understanding and application of DNA typing and nucleic acid amplification. His work continues to influence research and diagnostics in genetics.