Sohail Malik

Title: Sohail Malik: Innovator in Protein Production

Introduction

Sohail Malik is a notable inventor based in Englewood Cliffs, NJ (US). He has made significant contributions to the field of biotechnology, particularly in the production of human proteins.

Latest Patents

Sohail Malik holds a patent for the "High yield production of human apolipoprotein A1 in E. coli." This innovative method involves preparing apolipoprotein A1 in a T7 RNA polymerase/promoter bacterial expression system that optimizes yield and facilitates subsequent purification of the protein. The process includes cloning apolipoprotein A1 cDNA into an Escherichia coli plasmid containing a bacteriophage T7 RNA polymerase promoter. The recombinant plasmid is then transferred to an E. coli strain with inducible bacteriophage T7 RNA polymerase. This method allows for the isolation of apolipoprotein A1 from the culture, with preferred embodiments tagging the protein for easier purification.

Career Highlights

Sohail Malik is associated with the American Cyanamid Company, where he has applied his expertise in protein production. His work has led to advancements in the field, particularly in optimizing the yield of apolipoprotein A1.

Collaborations

Some of his notable coworkers include Florence Mahlberg and Sotirios K Karathanasis, who have contributed to his research endeavors.

Conclusion

Sohail Malik's innovative work in the production of human apolipoprotein A1 showcases his significant impact on biotechnology. His contributions continue to influence the field and enhance our understanding of protein production.