Robert T Schimke

Title: The Innovations of Robert T. Schimke

Introduction

Robert T. Schimke is a notable inventor based in Palo Alto, California. He has made significant contributions to the field of biotechnology, particularly in protein production and gene expression systems. With a total of 2 patents, his work has advanced the understanding and application of genetic engineering.

Latest Patents

One of Schimke's latest patents is focused on protein production at synthetic start sites. This patent outlines methods and compositions for producing heterologous proteins in a microorganism host, particularly bacteria, without the need for fusion to an endogenous protein. The heterologous gene can be inserted into a vector that includes an endogenous gene, with the heterologous gene preceded by a ribosomal binding site. This innovation demonstrates the functionality of a heterologous enzyme in a bacterial host.

Another significant patent by Schimke involves intronic overexpression vectors. This patent provides DNA constructs that utilize intronically positioned expression systems. In this system, one gene is a dominant gene, typically amplifiable, while the other encodes a sequence of interest. This approach achieves higher levels of co-expression compared to when the genes are ligated in tandem. Specifically, the gene of interest is inserted into the intron of a DHFR minigene, and the construct is transformed into a mammalian cell. The resulting transformants are then stressed with progressively increasing levels of methotrexate, leading to substantially increased levels of co-expression.

Career Highlights

Robert T. Schimke is affiliated with Leland Stanford Junior University, where he continues to contribute to research and innovation in biotechnology. His work has been instrumental in developing new methods for protein production and gene expression, which have broad applications in medicine and industry.

Collaborations

Some of Schimke's notable coworkers include John M. Abrams and Susan M. Thorpe. Their collaborative efforts have further enhanced the research output and innovation in their respective fields.

Conclusion

Robert T. Schimke's contributions to biotechnology through his patents and research at Leland Stanford Junior University highlight his role as a leading inventor in the field. His innovative approaches to protein production and gene expression continue to influence advancements in genetic engineering.