Richard M Eglen

Title: The Innovative Contributions of Richard M. Eglen

Introduction

Richard M. Eglen is a prominent inventor based in Los Altos, California, known for his significant contributions to the field of biotechnology. With a total of seven patents to his name, he has developed innovative methods that enhance our understanding of protein modifications and intracellular monitoring systems.

Latest Patents

One of his latest patents is focused on the analysis of intracellular modifications. This invention provides improved methods for determining the intracellular state of a protein and its modifications. By introducing a surrogate fusion protein that comprises a member of an enzyme fragment complementation complex and a target protein, researchers can expose transformed cells to environmental changes, such as candidate drugs. The cells are then lysed, and the lysate is separated into fractions using gel electrophoresis. The proteins are transferred to a membrane via Western blotting, where the bands are developed using the other member of the enzyme fragment complementation complex, resulting in a detectable signal. This method is noted for its high sensitivity and capability to observe modifications of the target protein.

Another significant patent is the genetic construct intracellular monitoring system. This system features an enzyme donor fused to a surrogate of a mammalian protein of interest, allowing the fusion protein to retain the function of the natural protein. The invention includes a vector with a regulatory region functional in a mammalian host cell, a sequence encoding the enzyme donor linked to a multiple cloning site, an enzyme acceptor protein, and a substrate for the enzyme formed by the enzyme donor and acceptor.

Career Highlights

Richard M. Eglen has had a distinguished career, working with notable companies such as Syntex Inc. and Discoverx Corporation. His work in these organizations has contributed to advancements in biotechnology and pharmaceutical research.

Collaborations

Throughout his career, Richard has collaborated with esteemed colleagues, including Robin D. Clark and William L. Smith. These partnerships have fostered innovation and the development of groundbreaking technologies in the field.

Conclusion

Richard M. Eglen's contributions to biotechnology through his patents and collaborations have significantly advanced our understanding of protein behavior and monitoring systems. His innovative work continues to impact the scientific community and drive future research.