Richard J Obiso

Title: Richard J. Obiso and Real-Time Detection of Viable Bacterial Spores.

Introduction

Richard J. Obiso is an inventor associated with Gaithersburg, Maryland, and the University of California. His patent work addresses the detection of viable bacterial spores, a challenge relevant to identifying biological contamination.

Latest Patents

Obiso is credited with 1 patent, titled “Real Time Viability Detection of Bacterial Spores.” The invention describes a process that places a sample in a germination medium long enough for viable spores to begin germination. The sample is then mixed with a lanthanide solution that can form a fluorescent complex with dipicolinic acid, a substance associated with bacterial spores, and measured for the presence of that compound.

The patent also describes a detection system with a germination chamber connected to sample, germinant, and bleach chambers. After filtration, the sample passes to a detection chamber, where a fluorescence-promoting metal, a spectral excitation source, and an emission-measurement mechanism support detection. The system also includes a waste chamber. A germination mixture containing L-alanine, L-asparagine, and D-glucose is described as a means of promoting viable spores’ commitment to germination.

Career Highlights

The patent reflects an applied approach to spore detection: rather than merely identifying spore material, the method is designed to determine whether spores are viable by prompting a germination response and measuring a related signal. The supplied information associates Obiso with the University of California.

Collaborations

The listed coworkers associated with Obiso are Laura A. Vanderberg and Timothy J. Herdendorf. The available information does not specify their individual roles in the patent or their broader professional relationship with Obiso.

Conclusion

Richard J. Obiso’s patent combines germination chemistry and fluorescence-based measurement to detect viable bacterial spores, highlighting a practical innovation in biological detection.