Rebecca Kucera

Title: Innovations by Rebecca Kucera in DNA Assembly

Introduction

Rebecca Kucera is an accomplished inventor based in Hamilton, MA (US). She has made significant contributions to the field of molecular biology, particularly in the area of DNA assembly. With a total of two patents to her name, Kucera's work focuses on improving the efficiency and fidelity of assembling DNA fragments.

Latest Patents

Kucera's latest patents include "Ordered assembly of multiple DNA fragments" and "Compositions and methods for improved in vitro assembly of polynucleotides." The first patent describes a synthetic self-complementary oligonucleotide that features a double-stranded region with a binding sequence for PaqCI. This oligonucleotide is designed with unligatable 3' and 5' ends that cannot be cleaved by PaqCI, allowing for precise assembly in a reaction mixture that may include ligases and repair enzymes. The second patent outlines novel methods and compositions that enhance the assembly of large DNA fragments, utilizing a computer system that integrates ligation data to optimize synthetic overhangs. This innovation has been exemplified by the successful assembly of a viral genome and a bacterial operon from multiple fragments.

Career Highlights

Kucera is currently employed at New England Biolabs, Inc., where she continues to advance her research in DNA assembly techniques. Her work has been pivotal in developing methods that improve the accuracy and efficiency of genetic engineering processes.

Collaborations

Throughout her career, Kucera has collaborated with notable colleagues such as Gregory Lohman and Vladimir Potapov. These partnerships have contributed to the success of her innovative projects and patents.

Conclusion

Rebecca Kucera's contributions to the field of DNA assembly are noteworthy and demonstrate her commitment to advancing molecular biology. Her innovative patents and ongoing work at New England Biolabs, Inc. continue to impact the scientific community positively.