Company Filing History:
Years Active: 1996-1998
Title: Innovations of Raymond D Harris
Introduction
Raymond D Harris is a notable inventor based in Falkirk, GB. He has made significant contributions to the field of biotechnology, particularly in the area of restriction-modification systems. With a total of 2 patents, his work has advanced the understanding and application of genetic engineering techniques.
Latest Patents
One of his latest patents is the "Cloned SstI/SacI restriction-modification system." This invention discloses the cloning and expression of the SstI/SacI restriction-modification system in Escherichia coli. The SstI methylase gene was cloned using methylase selection, known as the 'Hungarian Trick,' and the orientation and boundaries of the gene were established. The level of methylation of the host was significantly improved by expressing the SstI methylase from the strong RPSL promoter. By using the SstI methylase clone as a probe, a chromosomal map was generated in the area of the gene for the SstI methylase. DNA downstream from the methylase was cloned and found to contain the gene for the SstI endonuclease. The orientation of the SstI endonuclease was established, and a strong promoter was placed close to the SstI endonuclease gene by generating nested deletions. Through these methods, an E. coli strain was constructed that produced high levels of SstI endonuclease. Additionally, the SacI locus in the S. achromogenes chromosome was found to be identical to the SstI locus in S. stanford in all measured respects.
Career Highlights
Raymond D Harris is currently associated with Life Technologies Corporation, where he continues to innovate and contribute to advancements in biotechnology. His work has been instrumental in developing tools that enhance genetic research and applications.
Collaborations
He has collaborated with notable coworkers, including Mary C Longo and Michael D Smith, who have contributed to his research endeavors.
Conclusion
Raymond D Harris is a distinguished inventor whose work in biotechnology has led to significant advancements in genetic engineering. His contributions, particularly in the development of restriction-modification systems, continue to impact the field positively.