R Bruce Moffett

Title: R Bruce Moffett: Innovator in DNA Polymerase Technology

Introduction

R Bruce Moffett is a notable inventor based in Shaker Heights, OH (US). He has made significant contributions to the field of biotechnology, particularly in the development of thermostable DNA polymerases. His work has implications for various applications in molecular biology and genetic research.

Latest Patents

Moffett holds a patent for a novel enzymatically active DNA polymerase. This polymerase consists of between 540 and 582 amino acids and features a tyrosine at a position equivalent to position 667 of Taq DNA polymerase. Notably, this polymerase lacks 5' to 3' exonuclease activity and exhibits at least 95% homology in its amino acid sequence to the DNA polymerases of Thermus aquaticus, Thermus flavus, or Thermus thermophilus. Additionally, it forms a single polypeptide band on an SDS PAGE. This innovation is crucial for enhancing the efficiency and reliability of DNA amplification processes.

Career Highlights

Moffett is currently associated with Amersham Life Science, Inc., where he continues to advance research in biotechnology. His expertise in enzymatic processes has positioned him as a key figure in the development of tools that facilitate genetic analysis and manipulation.

Collaborations

Throughout his career, Moffett has collaborated with esteemed colleagues, including Maria Davis and Carl Williams Fuller. These partnerships have fostered a collaborative environment that promotes innovation and the sharing of ideas in the scientific community.

Conclusion

R Bruce Moffett's contributions to the field of DNA polymerase technology exemplify the impact of innovative thinking in biotechnology. His patent and ongoing work continue to influence advancements in genetic research and applications.