Company Filing History:
Years Active: 1991-1994
Title: Otello Protasi: Innovator in Interferon Purification
Introduction
Otello Protasi is a notable inventor based in Siena, Italy. He has made significant contributions to the field of biotechnology, particularly in the purification of human recombinant gamma interferon. With a total of 2 patents, Protasi's work has advanced the methods used in the extraction and purification of vital biological substances.
Latest Patents
Protasi's latest patents include a process for the extraction and purification of human recombinant gamma interferon. This innovative method involves suspending recombinant host cells in a buffer solution with a pH ranging from 6.0 to 8.5, optionally complemented with an osmoprotector. The process ensures that the cells are disrupted under conditions that do not activate endocellular proteases. The resulting pellet containing insoluble HuIFN-gamma is then separated and solubilized using phosphate buffer, followed by purification through various chromatographic techniques. This method allows for the production of non-degraded HuIFN-gamma, maintaining its biological activity and chemical-physical properties while achieving high yield and purity.
Another patent focuses on a method for the purification of crude human interferon from solutions containing it. This method enhances the efficiency of obtaining pure interferon, which is crucial for therapeutic applications.
Career Highlights
Otello Protasi has been associated with Sclavo Spa, a company known for its research and development in the field of biotechnology. His work at Sclavo Spa has positioned him as a key figure in the advancement of interferon purification techniques.
Collaborations
Throughout his career, Protasi has collaborated with esteemed colleagues such as Paolo Rappuoli and Donatella Mannucci. These collaborations have further enriched his research and contributed to the success of his innovative projects.
Conclusion
Otello Protasi's contributions to the field of biotechnology, particularly in the purification of human recombinant gamma interferon, highlight his role as an influential inventor. His patents reflect a commitment to advancing scientific knowledge and improving therapeutic processes.