Noriyuki Hongo

Title: Noriyuki Hongo: Innovator in Glutamate Dehydrogenase Research

Introduction

Noriyuki Hongo is a notable inventor based in Tsuruga, Japan. He has made significant contributions to the field of biochemistry, particularly in the study of glutamate dehydrogenase. With a total of 2 patents, Hongo's work has implications for various biochemical applications.

Latest Patents

Hongo's latest patents focus on a novel glutamate dehydrogenase derived from the genus Pseudomonas. This enzyme is specific to L-glutamic acid and requires NAD⁺ and NADH as coenzymes. It does not act on NADP⁺ and NADPH, demonstrating an optimum pH of 10.5-11.5 for oxidative deamination. The enzyme exhibits a pH stability of 5-10 at 25°C for 20 hours and an optimum temperature of about 60°C for oxidative deamination. Its molecular weight is approximately 280,000 as determined by gel filtration or about 41,000 via SDS-PAGE, and it is not activated by ADP. Additionally, he has developed a reagent composition for measuring ammonia, which includes a glutamate dehydrogenase that is NADH-dependent, α-ketoglutaric acid or a salt thereof, and NADH. This composition can be further enhanced by the addition of urease or creatinine deiminase.

Career Highlights

Hongo is associated with Toyo Boseki Kabushiki Kaisha, where he continues to advance his research. His work has been instrumental in understanding the biochemical properties of glutamate dehydrogenase and its applications in various fields.

Collaborations

Hongo has collaborated with notable colleagues such as Shinichi Teshima and Shizuo Hattori. Their combined efforts have contributed to the advancements in the study of glutamate dehydrogenase.

Conclusion

Noriyuki Hongo's innovative research in glutamate dehydrogenase has paved the way for new biochemical applications. His contributions are significant in the field and continue to influence ongoing research.