Michael R Hollaway, Deceased

Title: Michael R Hollaway: Innovator in Enzyme Detection

Introduction

Michael R Hollaway was a notable inventor recognized for his contributions to the field of enzyme detection. His innovative work has left a lasting impact on biotechnology, particularly in the development of sensitive assays for hydrolase enzymes.

Latest Patents

Hollaway held a patent for an "Amplification assay for hydrolase enzymes." This invention involves the use of novel substituted FAD substrates that are hydrolyzed to FAD by the enzyme being detected. The FAD is then combined with an apoenzyme to form a holoenzyme, which initiates a reaction leading to a detectable product. When the hydrolase to be detected is phosphatase, the novel substrate is a phosphorylated derivative of FAD. A suitable apoenzyme for this process is apo-glucose oxidase, which provides exceptional sensitivity. Additionally, apo-D-amino acid oxidase can be utilized in a 'single pot' assay system.

Career Highlights

Hollaway was associated with London Biotechnology Limited, where he contributed significantly to research and development in enzyme assays. His work has been instrumental in advancing the methodologies used in biochemical analysis.

Collaborations

Throughout his career, Hollaway collaborated with notable colleagues, including Brian R Rabin and Stuart Harbron. These collaborations helped to enhance the quality and reach of his innovative work.

Conclusion

Michael R Hollaway's contributions to enzyme detection through his patented innovations have made a significant impact in the field of biotechnology. His legacy continues to influence research and development in enzyme assays today.