This inventor holds 5 USPTO granted patents. Top assignee: Becton Dickinson and Company Limited. Active years: 1997-1998.
Location History:
- San Jose, CA (US) (1997)
- Durham, NC (US) (1997 - 1998)
Company Filing History:
Years Active: 1997-1998
Title: Innovations in Nucleic Acid Detection by Mark Van Cleve
Introduction
Mark Van Cleve is a notable inventor based in Durham, NC (US), recognized for his contributions to the field of nucleic acid detection. He holds a total of 5 patents, showcasing his innovative approach to amplifying nucleic acid target sequences.
Latest Patents
One of his latest patents involves the detection of nucleic acids in cells through a method known as Thermophilic Strand Displacement Amplification (tSDA). This technique allows for the amplification of nucleic acid target sequences in situ within cells, whether in suspension, on slides, or in tissues. The method preserves excellent specimen morphology and enables selective amplification of both DNA and RNA targets. Furthermore, tSDA is compatible with immunochemical techniques, allowing for simultaneous amplification of target sequences and immunological staining on the same specimen.
Another significant patent by Van Cleve focuses on strand displacement amplification using thermophilic enzymes. This method can be performed over a broad temperature range, specifically from 37°C to 70°C, with a preferred range of 50°C to 70°C. His research has demonstrated that certain thermophilic restriction endonucleases can effectively nick the hemimodified recognition sites required for strand displacement amplification. Additionally, specific thermophilic polymerases can extend from the nick while displacing the downstream strand. The advantages of thermophilic SDA include improved specificity and efficiency, reduced nonspecific background amplification, and potentially enhanced yields of amplification products.
Career Highlights
Mark Van Cleve is currently associated with Becton Dickinson and Company Limited, where he continues to advance his research in nucleic acid detection technologies. His work has significantly impacted the field, providing innovative solutions for researchers and clinicians alike.
Collaborations
He has collaborated with notable colleagues, including Kenton L Lohman and Natalie V Ostrerova, contributing to the advancement of their shared research interests.
Conclusion
Mark Van Cleve's innovative work in nucleic acid detection through thermophilic strand displacement amplification represents a significant advancement in the field. His contributions continue to influence research and clinical practices, showcasing the importance of innovation in scientific discovery.