This inventor holds 2 USPTO granted patents. Top assignee: Alpha Therapeutic Corporation. Active years: 1997-1999.
Company Filing History:
Years Active: 1997-1999
Title: Mark Lepe: Innovator in Protein Purification
Introduction
Mark Lepe is a notable inventor based in West Covina, California. He has made significant contributions to the field of protein purification, holding 2 patents that showcase his innovative approaches. His work primarily focuses on enhancing the yield and purity of alpha-1-proteinase inhibitor (alpha-1-PI).
Latest Patents
Mark Lepe's latest patents include a process for increasing the yield of a protein that has been subjected to purification. This process involves providing an impure protein fraction containing alpha-1-PI, which is suspended in an aqueous solution at pH 6. Insoluble proteins are recovered and resuspended in an aqueous solution at pH 8.5. Polyethylene glycol (PEG) is added to precipitate alpha-2 proteins. Subsequently, zinc chloride (ZnCl2) is introduced to the PEG supernatant to precipitate crude alpha-1-PI. The crude alpha-1-PI is then resolubilized and applied to an anion-exchange medium, from which a fraction comprising alpha-1-PI is recovered. The purified alpha-1-PI exhibits a specific activity of about 1.0 units/OD280.
Another significant patent by Mark Lepe is directed towards a process for separating alpha-1-proteinase inhibitor from COHN IV1. This process follows a similar methodology, emphasizing the importance of precise conditions for effective protein purification.
Career Highlights
Mark Lepe is currently associated with Alpha Therapeutic Corporation, where he continues to advance his research in protein purification. His work has been instrumental in developing methods that enhance the efficiency and effectiveness of protein extraction and purification processes.
Collaborations
Throughout his career, Mark has collaborated with esteemed colleagues such as Duk Sung Hwang and Kazuo Takechi. These partnerships have contributed to the innovative advancements in his field.
Conclusion
Mark Lepe's contributions to protein purification through his patented processes highlight his role as an influential inventor in the biotechnology sector. His work not only advances scientific understanding but also has practical applications in medicine and research.