Mark L Stolowitz

Title: Mark L Stolowitz: Innovator in Peptide Sequencing

Introduction

Mark L Stolowitz is a notable inventor based in Long Beach, California, with a significant contribution to the field of peptide sequencing. He holds a total of six patents, showcasing his innovative approaches to protein analysis and sequencing methods.

Latest Patents

Among his latest patents, Stolowitz has developed a "Method of thioacylation peptide sequencing with alcoholysis." This sequencing method exploits the thioacylation degradation of polypeptides and proteins. The process involves the reaction of the N-terminal amino acid of a polypeptide with an excess of a thioacylating reagent. After ensuring quantitative coupling and removing excess reagent, the N-thioacyl polypeptide undergoes acid cleavage, resulting in a 2-substituted-5(4H)-thiazolinone of the N-terminal amino acid. The subsequent addition of a large excess of an aliphatic primary or secondary alcohol yields the corresponding N-thioacyl amino acid ester, a stable compound suitable for chromatographic separation and detection by high-pressure liquid chromatography.

Another significant patent is the "Method of thioacylation peptide sequencing with acylation." This protein micro sequencing method is designed for use in conjunction with the thioacylation degradation of polypeptides and proteins. The process similarly involves the reaction of the N-terminal amino acid with a thioacylating reagent, followed by acid cleavage to afford a 2-substituted-5(4H)-thiazolinone. After removing excess acid, the thiazolinone is reacted with a small excess of a fluorescent or enhanced ultraviolet absorbance reagent, yielding a detectable 5-O-acyl-2-(substituted)thiazole derivative at extremely low concentrations.

Career Highlights

Stolowitz has worked with several prominent companies, including Bio-Affinity Systems, Inc. and Varian Associates, Inc. His work in these organizations has contributed to advancements in peptide sequencing technologies.

Collaborations

Throughout his career, Stolowitz has collaborated with notable colleagues such as Amy G Taketomo and Beth A Paape. These collaborations have further enriched his contributions to the field of peptide sequencing.

Conclusion

Mark L Stolowitz is a distinguished inventor whose innovative methods in peptide sequencing have made a significant impact in the