Company Filing History:
Years Active: 2024-2025
Title: Longyu Wang: Innovator in DNA Synthesis and Assembly
Introduction
Longyu Wang is a prominent inventor based in Wuhan, China. He has made significant contributions to the field of biotechnology, particularly in the synthesis and assembly of large fragment DNA. With a total of 2 patents, his work is paving the way for advancements in genetic engineering.
Latest Patents
Wang's latest patents include an efficient synthesis and assembly method for large fragment DNA based on programmable nuclease Argonaute. This innovative method involves constructing and treating antibiotic resistance gene reconstructed vectors with linearization. It also includes dividing a target DNA into multiple small DNA fragments, synthesizing these fragments, and loading them onto the antibiotic resistance gene reconstructed vectors. The method combines the SLIC technique with a resistance gene reconstruction strategy, allowing for the assembly of 5-6 small fragments in a single reconstruction, which is both efficient and time-saving. Additionally, this approach avoids mutations caused by PCR, eliminating the need for a second sequencing, thus saving time and costs.
Another notable patent is related to the PfAgo mutant protein, which exhibits mesophilic target nucleic acid cleavage activity. This mutant protein has specific amino acid mutations that enhance its activity within a temperature range of 30-95 degrees Celsius. This advancement effectively expands the application range of prokaryotic Argonaute (pAgo) proteins, making them more versatile in various biotechnological applications.
Career Highlights
Longyu Wang is affiliated with Hubei University, where he continues to engage in groundbreaking research. His work has garnered attention in the scientific community, contributing to the advancement of genetic engineering techniques.
Collaborations
Wang collaborates with notable colleagues, including Lixin Ma and Wanping Chen, who share his passion for innovation in biotechnology.
Conclusion
Longyu Wang's contributions to DNA synthesis and assembly represent a significant leap forward in biotechnology. His innovative methods and collaborative efforts are shaping the future of genetic engineering.