Lisa Hochrein

Title: Innovations of Lisa Hochrein in CRISPR Technology

Introduction

Lisa Hochrein is an accomplished inventor based in Pasadena, California. She has made significant contributions to the field of biotechnology, particularly in the development of innovative CRISPR technologies. With a total of three patents to her name, Hochrein is at the forefront of advancements that have the potential to revolutionize gene editing and therapeutic applications.

Latest Patents

One of Hochrein's latest patents focuses on allosteric conditional guide RNAs for cell-selective regulation of CRISPR/Cas. Programmable guide RNAs (gRNAs) play a central role in the CRISPR revolution sweeping biology and medicine by directing the function of a Cas protein effector to a target gene of choice. To achieve programmable control over regulatory scope, the activity of a conditional guide RNA (cgRNA) depends on the presence or absence of an RNA trigger, allowing for cell-selective regulation of CRISPR/Cas function. Unlike a standard gRNA, a cgRNA is programmable at multiple levels, with the target-binding sequence controlling the target of Cas activity (edit, silence, induce, or bind a gene of choice) and the trigger-binding sequence controlling the scope of Cas activity. cgRNA mechanisms that are allosteric allow for independent design of the target and trigger sequences, providing the flexibility to select the regulatory target and scope independently. Disclosed herein are allosteric cgRNA mechanisms for both ON→OFF logic (conditional inactivation by an RNA trigger) and OFF→ON logic (conditional activation by an RNA trigger). Allosteric cgRNAs enable restriction of CRISPR/Cas function to a desired cell type, tissue, organ, or disease state. Allosteric cgRNAs provide a versatile platform for cell-selective and tissue-selective research tools, biotechnologies, diagnostics, and therapeutics.

Career Highlights

Hochrein's work at the California Institute of Technology has positioned her as a leading figure in the field of genetic engineering. Her innovative approaches to CRISPR technology have garnered attention and respect from her peers in the scientific community.

Collaborations

Some of her notable coworkers include Niles A. Pierce and Mikhail H. Hanewich-Hollatz, who have collaborated with her on various projects related to CRISPR and gene editing technologies.

Conclusion

Lisa Hochrein