Linda K Cragle

Title: Innovations in Immunoassay by Linda K. Cragle

Introduction

Linda K. Cragle is an accomplished inventor based in San Diego, California, with a remarkable track record of three patents. Her work primarily focuses on developing innovative methods in immunoassays, which play a crucial role in various biochemical analyses.

Latest Patents

Among Linda's latest contributions, one of her notable patents is a "Method to achieve a linear standard curve in a sandwich immunoassay." This invention presents an effective methodology for obtaining an actual linear standard curve in a sandwich type of immunoassay. In this process, a first antibody is bound to an insoluble support, while a second unbound labeled antibody complexes with the antigen in a test sample to form an insoluble antibody:antigen:labeled antibody complex, which can then be detected.

Linda's approach involves adding unbound unlabelled first and/or second antibodies to divert excess antigen away from the desired end-product complex. This process not only optimizes the reaction but also makes the antigen of interest the rate-limiting factor, resulting in a pseudo first-order reaction that produces a true linear standard curve.

Another significant patent is focused on "Immunoassays and kits for use therein, which include low affinity." This patent describes an innovative immunoassay for assaying an antigenic substance in fluid, characterized by involving multiple antibody entities to avoid hook effects. The detailed approach ensures accuracy and reliability in detecting various antigens, thereby enhancing the field of immunoassays significantly.

Career Highlights

Linda K. Cragle has built her distinguished career at Beckman Instruments, Inc., where she applies her expertise in immunoassay development. Her contributions have not only advanced the company’s innovations but also set new standards in the industry for biochemical analysis.

Collaborations

Throughout her career, Linda has collaborated with esteemed colleagues such as Shih-Yun Lee and Ker-Kong Tung. These collaborations have fostered a creative environment that encourages the exchange of ideas, enhancing the innovative process necessary for developing cutting-edge technologies.

Conclusion

Linda K. Cragle stands out as a pioneer in the field of immunoassays. Her inventive methodologies have made significant impacts within the scientific community. As she continues to innovate at Beckman Instruments, her patents serve as a testament to her expertise and dedication to advancing technology in biochemical analysis.