Leow Thean Chor

Title: Leow Thean Chor: Innovator in Biotechnological Advancements

Introduction

Leow Thean Chor is a notable inventor based in Selangor, Malaysia. He has made significant contributions to the field of biotechnology, particularly in the production of enzymes from novel bacterial strains. His work has implications for various industrial applications, especially in the processing of palm oil.

Latest Patents

Leow Thean Chor holds a patent for a biologically pure culture of Strain T1 bacteria isolated from Palm Oil Mill Effluent. This strain is capable of producing a thermostable lipase T1 gene. The production process involves an aerobic, gram-positive, endospore-forming, rod-shaped bacterium. The genomic DNA of this strain has a G+C content of 52.6%. Through physiological data, phenotypic traits, and molecular analysis, strain T1 has been classified as a novel species within its genus. The thermostable T1 lipase gene was subcloned into the pGEX-4T1 vector, both with and without a signal peptide in a prokaryotic system. The signal peptide, consisting of 28 amino acid residues, alters the conformation of the GST moiety, preventing it from binding to the affinity glutathione Sepharose column. The expression and purification process through single-step affinity chromatography yielded a specific activity of 292.929 U/mg, with 72.55% of the fusion lipase recovered from crude cell lysate. The mature T1 lipase was successfully purified, achieving a final recovery of 51.49% and a specific activity of 959.033 U/mg.

Career Highlights

Leow Thean Chor is affiliated with Universiti Putra Malaysia, where he conducts his research and development activities. His work has garnered attention for its innovative approach to enzyme production, which is crucial for enhancing industrial processes.

Collaborations

Leow has collaborated with notable colleagues, including Raja Noor Zaliha Abd Rahman and Abu Bakar Salleh. These collaborations have contributed to the advancement of research in the field of biotechnology.

Conclusion

Leow Thean Chor's contributions to biotechnology, particularly through his patented work on the thermostable lipase T1 gene, highlight his role as an innovator in the field. His research not only advances scientific knowledge but also has practical applications