Kenneth R Tometsko

Title: Kenneth R Tometsko: Innovator in Flow Cytometry

Introduction

Kenneth R Tometsko is a notable inventor based in Rochester, NY (US). He has made significant contributions to the field of flow cytometry, particularly in the enumeration of micronucleated erythrocyte populations. With a total of three patents to his name, Tometsko's work has advanced the understanding and analysis of blood cell populations.

Latest Patents

One of Kenneth R Tometsko's latest patents is a method for the enumeration of micronucleated erythrocyte populations. This innovative approach utilizes a single laser flow cytometric method to analyze micronuclei in erythrocyte populations. The process begins with obtaining a sample of peripheral blood or bone marrow, which is then fixed. Reticulocytes in the fixed samples are treated simultaneously with RNAse and a fluorescent labeled antibody that specifically binds to surface markers for erythroblasts and reticulocytes. The erythrocyte populations are stained with a nucleic acid stain that highlights DNA representing micronuclei, if present. The stained erythrocyte populations are exposed to a laser beam of appropriate excitation wavelength, producing fluorescent emissions. These emissions, along with light scatter from the erythrocyte populations, are detected by the flow cytometer, allowing for the calculation of specific erythrocyte populations in the sample.

Career Highlights

Kenneth R Tometsko is currently employed at Litron Laboratories Ltd., where he continues to develop and refine his innovative methods in flow cytometry. His expertise in this area has positioned him as a key figure in the advancement of hematological analysis techniques.

Collaborations

Tometsko has collaborated with notable colleagues such as Stephen D Dertinger and Dorothea K Torous. These partnerships have further enhanced the research and development efforts in the field of flow cytometry.

Conclusion

Kenneth R Tometsko's contributions to the field of flow cytometry through his innovative patents and collaborations have significantly impacted the analysis of blood cell populations. His work continues to influence advancements in medical research and diagnostics.