Company Filing History:
Years Active: 2003-2004
Title: Innovations of Julian A J Hanak
Introduction
Julian A J Hanak is a notable inventor based in Macclesfield, GB. He has made significant contributions to the field of gene therapy and recombinant protein production. With a total of two patents to his name, Hanak's work is recognized for its innovative approaches to plasmid stabilization and DNA production.
Latest Patents
Hanak's latest patents include a system for plasmid stabilization, which utilizes a novel method of repressor titration. This system is designed for the maintenance of a plasmid that is useful in gene therapy and the production of recombinant proteins. The system involves a transformed host cell containing a plasmid with an operator that can bind to a repressor expressed in trans. Additionally, it includes a first chromosomal gene encoding the repressor and a second chromosomal gene essential for cell growth. The plasmid is maintained in sufficient numbers to titrate the repressor, allowing for the expression of the essential gene and promoting cell growth.
Another significant patent is a method for plasmid DNA production and purification. This scalable method focuses on producing highly purified plasmid DNA by growing plasmid-containing cells to a high biomass during exponential growth. The cells are lysed by carefully raising the pH of the culture to a controlled value, which denatures chromosomal DNA while allowing plasmid DNA to renature. This method has been developed for the production of pharmaceutical-grade DNA for use in both in vivo and ex vivo gene therapy.
Career Highlights
Throughout his career, Hanak has worked with reputable companies such as Cobra Therapeutics Limited and Cobra Biologics Limited. His experience in these organizations has contributed to his expertise in the field of biotechnology and gene therapy.
Collaborations
Some of Hanak's notable coworkers include David R Thatcher and Anthony Hitchcock. Their collaborations have likely played a role in advancing the innovative projects they have worked on together.
Conclusion
Julian A J Hanak is a distinguished inventor whose work in plasmid stabilization and DNA production has made a significant impact in the field of gene therapy. His innovative patents and collaborations highlight his contributions to biotechnology.